Display More“In terms of the 20 degrees - I suppose that means the denaturing step is more efficient? What is the value and benefit of the lower temperature ?”
The value, it is that if the reaction works at 20°C without denaturation step, it could work at 37°C without denaturation step.
And 37°C is the body temperature.
What do you think about the RT-PCR primers used for Covid - and the potential for false positives?
Is there any good literature we can read on this?