Vitamin D and interferon-γ co-operate to increase the ACE-2 receptor expression in primary cultures of human thyroid cells

Vitamin D and interferon-γ co-operate to increase the ACE-2 receptor expression in primary cultures of human thyroid cells - Journal of Endocrinological Investigation

Background A more severe course of COVID-19 was associated with low levels of Vitamin D (VitD). Moreover in vitro data showed that VitD up-regulates the mRNA…

link.springer.com

Abstract

Background

A more severe course of COVID-19 was associated with low levels of Vitamin D (VitD). Moreover in vitro data showed that VitD up-regulates the mRNA of the Angiotensin Converting Enzyme 2 (ACE-2), the SARS-COV-2 receptor in different type of cells. ACE-2 is expressed in several type of tissues including thyroid cells, on which its mRNA was shown to be up-regulated by interferon-gamma (IFN-γ). The aim of the present study was to investigate if treatment with VitD alone or in combination with IFN-γ would increase ACE-2 both at mRNA and protein levels in primary cultures of human thyrocytes.

Materials and methods

Primary thyroid cell cultures were treated with VitD and IFN-γ alone or in combination for 24 h. ACE-2 mRNA levels were measured by Real-time Polymerase Chain Reaction (RT-PCR). The presence of ACE-2 on thyroid cell membrane was assessed by immunocytochemistry basally and after the previous mentioned treatments.

Results

ACE-2 mRNA levels increased after treatment with VitD and IFN-γ alone. The combination treatment (VitD + IFN-γ) showed an additive increase of ACE-2-mRNA. Immunocytochemistry experiments showed ACE-2 protein on thyroid cells membrane. ACE-2 expression increased after treatment with VitD and IFN-γ alone and further increased by the combination treatment with VitD + IFN-γ.

Conclusions

VitD would defend the body by SARS-COV2 both by regulating the host immune defense and by up-regulating of the expression of the ACE-2 receptor. The existence of a co-operation between VitD and IFN-γ demonstrated in other systems is supported also for ACE-2 up-regulation. These observations lead to an increased interest for the potential therapeutic benefits of VitD supplementation in COVID-19.

Quote from Fm1

By Peter A. McCullough, MD, MPH

Yep - that is what I mean FM1 - you are right on the money

Peter A. McCullough - Wikipedia

Summary -

• He is a cardiologist
• No expertise in vaccines
• Nor in antivirals
• Made false claims about ivermectin and hydrochloroquine
• Makes false claims about the mRNA vaccines

antivaxxer.

How do you diagnose:

(1) pontificates outside area of expertise

(2) no knowledge of vaccines

(3) hold various diverse non-conventional opinions about different topics in covid epidemic - only unifying factor being that they are antivaxxer memes

More on McCullough - who seems a real sucker for conspiracy memes:

Some of McCullough's public statements contributed to the spread of COVID-19 misinformation.^[4][5]

McCullough testified before a committee of the Texas Senate in March 2021, posted to YouTube by the Association of American Physicians and Surgeons, in which he made false claims about COVID-19 and COVID-19 vaccines, including that people under 50 years of age and survivors do not need the vaccine and that there is no evidence of asymptomatic spread of COVID-19.^[28]

Posted on the Canadian online video sharing platform Rumble, McCullough gave an interview in April 2021 to The New American, the magazine of the right-wing John Birch Society, in which he advanced anti-vaccination messaging, including falsely claiming huge numbers of fatalities attributed to the COVID-19 vaccines.^[23] In May 2021, McCullough gave an interview in which he made claims about COVID-19 and COVID-19 vaccines which were "inaccurate, misleading and/or unsupported by evidence", including that survivors cannot be re-infected and so do not require vaccination and that the vaccines are dangerous.^[29]

During television appearances, McCullough has contradicted public health recommendations, including when asked about the aggressive spread of COVID-19 among children, by suggesting that healthy persons under 30 had no need for a vaccine,^[30][48] and when asked about the relative merits of vaccination-induced immunity versus "natural" (survivor) immunity, by disputing the necessity of vaccinations to achieve herd immunity.^{[4][22][49][50]} In December 2021, McCullough appeared on the Joe Rogan Experience promoting debunked conspiracy theories and misinformation (e.g. the COVID-19 pandemic was planned, the spike protein causes cell death, medical authorities are conspiring to illegitimately suppress hydroxychloroquine and ivermectin).^[26][27][51]

Ahhh... Found it.

Not surprising he is an antivaxxer, he is a member of the Association of American Physicians and Surgeons .

Keeps bad company.

Ok, so I'll leave the antivaxxers here (an those not antivaxxers who just like reading antivaxxer memes endlessly recirculated) to their fun!

Quote from THHuxleynew

There is no reason at all why a vaccine cannot produce better immunity than infection.

Our clown again mixes up vaccine with RNA gene therapy ...Grööööll miauuuuuuuuuuuuu

Quote from Wyttenbach

Our clown

The Imperial clown should confine itself to electrical circuits..

has limited knowledge of biology.. like Gates

not a cardiologist but a circuitologist

Natural immunity beats RNA gene therapy

BNT162b2 etc anytime

even with slanted research

"Vaccine protection against infection waned with time after the second dose, whereas natural immunity showed little waning in protection for at least 8 months after the primary infection. However, onset of the omicron wave led to large increases in the incidence of reinfections in the natural infection cohort and the incidence of infections in the vaccinated cohorts. Nonetheless, even during the omicron wave, natural infection was associated with lower incidence than BNT162b2 and mRNA-1273 vaccination.

Protection from previous natural infection compared with mRNA vaccination against SARS-CoV-2 infection and severe COVID-19 in Qatar: a retrospective cohort study

Previous natural infection was associated with lower incidence of SARS-CoV-2 infection, regardless of the variant, than mRNA primary-series vaccination.…

www.thelancet.com

Quote from THHuxleynew

More on McCullough - who seems a real sucker for conspiracy memes:

Some of McCullough's public statements contributed to the spread of COVID-19 misinformation.^[4][5]

McCullough testified before a committee of the Texas Senate in March 2021, posted to YouTube by the Association of American Physicians and Surgeons, in which he made false claims about COVID-19 and COVID-19 vaccines, including that people under 50 years of age and survivors do not need the vaccine and that there is no evidence of asymptomatic spread of COVID-19.^[28]

Posted on the Canadian online video sharing platform Rumble, McCullough gave an interview in April 2021 to The New American, the magazine of the right-wing John Birch Society, in which he advanced anti-vaccination messaging, including falsely claiming huge numbers of fatalities attributed to the COVID-19 vaccines.^[23] In May 2021, McCullough gave an interview in which he made claims about COVID-19 and COVID-19 vaccines which were "inaccurate, misleading and/or unsupported by evidence", including that survivors cannot be re-infected and so do not require vaccination and that the vaccines are dangerous.^[29]

During television appearances, McCullough has contradicted public health recommendations, including when asked about the aggressive spread of COVID-19 among children, by suggesting that healthy persons under 30 had no need for a vaccine,^[30][48] and when asked about the relative merits of vaccination-induced immunity versus "natural" (survivor) immunity, by disputing the necessity of vaccinations to achieve herd immunity.^{[4][22][49][50]} In December 2021, McCullough appeared on the Joe Rogan Experience promoting debunked conspiracy theories and misinformation (e.g. the COVID-19 pandemic was planned, the spike protein causes cell death, medical authorities are conspiring to illegitimately suppress hydroxychloroquine and ivermectin).^[26][27][51]

Ahhh... Found it.

Not surprising he is an antivaxxer, he is a member of the Association of American Physicians and Surgeons .

Keeps bad company.

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So he's a bad guy because wiki says so. Jesus you are pathetic

Quote from Fm1

So he's a bad guy because wiki says so.

Probably knows a tad more medicine than an anonymous circuitous circuitologist

Peter A McCullough, MD, MPH

Truth for Health Foundation - Cited by 68.339 - Internal Medicine - Cardiology - Nephrology

scholar.google.com

Quote from Alan Smith

Britain has endured a decade of early deaths. Why?

The mystery of 250,000 dead Britons

Mar 9th 2023
Share

In recent years Britain has been hit by one health crisis after another. First came the covid-19 pandemic—then backlogs in health and social care that the coronavirus exacerbated, and a long winter of strikes and overwhelmed emergency departments. But in the background, long before the pandemic hit, an even more disturbing story has been unfolding. Britain has endured a grim decade during which perhaps a quarter of a million people died younger than expected. Listen to this story. Enjoy more audio and podcasts on iOS or Android.

By our calculations, that is the number of extra deaths Britain has suffered, compared with similar countries such as France and Denmark. The reason is that, in the early 2010s, life expectancy stalled in Britain compared with long-run trends and other countries. This slowdown in life expectancy struck all age groups, not just the elderly. And it disproportionately affected the poor. If you travel just 10km (six miles) from the poshest part of Kensington in London to New Cross Gate, life expectancy for men falls by a staggering 18 years, from 92 to 74. The burden these deaths place on the living is not just weighed in grief. When more people are dying and life expectancy is stagnating, a greater number of people are also living in ill health.

Life expectancy in Britain, as in almost all other rich countries, had been rising for nearly two centuries. But something went wrong in the early 2010s. Life expectancy at birth today, at 81, is just eight weeks longer than it was in 2011. In a best-case scenario, in which the pace of improvement between 1980 and 2011 had been sustained, life expectancy today would have been over 83. By The Economist’s calculations, that is no minor difference: it implies that between 2012 and 2022 approximately 700,000 Britons died sooner than they might have.

Two features make this figure even more worrying. Death comes mostly when people are old. But the slowdown in life expectancy has occurred across all age groups. Mortality rates have stalled for infants, and risen among young adults and the middle-aged. Death rates for 30- to 49-year-olds have steadily increased in Britain since around 2012, in sharp contrast with neighbouring countries.

Although the deaths have been spread across generations, they have not been spread across the income spectrum. Life expectancy has fallen among the poorest in society but risen for the richest. A poor English girl could on average expect to live 6.8 years less than a rich girl in 2011, but 7.7 less in 2017. For boys, the gap increased from 9.1 to 9.5 years over the same period.

The combined effect of the pandemic and global demographic trends can explain only some of Britain’s missing multitude. Though other rich countries have also experienced slowdowns, Britain has done the worst out of a cohort of its European peers. After stripping out the effects attributable to covid and the broad European slowdown from the toll of 700,000, you are still left with those 250,000 unexplained deaths.

Working out what has gone wrong is not easy. In America, where life expectancy has fallen even more sharply in recent years, “deaths of despair” from drugs, alcohol and suicide have done the most harm. The same is true for Scotland, where drug deaths have more than doubled in a decade; Dundee is now the drug-death capital of Europe. Yet although a similar problem may be brewing in England and Wales, the rate of drug deaths is nearly four times higher in Scotland.

The recent struggles of the National Health Service (nhs) have played their part. Hospital waits of record lengths and a crisis in primary care jeopardise timely treatment. But delays in medical care cannot explain all the extra deaths, especially before the pandemic. Besides, the greatest improvements in life expectancy come not from treatment but from better diagnosis and prevention, and wider prosperity. This is where Britain appears to have fallen short. It could do much better in all three.

First, diagnosis. Poorer Britons are 20% more likely to be diagnosed with cancer at a later stage, when the disease is more complex and expensive to treat. Having more nhs diagnostic centres would help, as well as cutting the pandemic-related backlog. Prescribing more statins for those at risk of heart attack or stroke would be good, too. Both treatments are cheap and cost-effective, and are recommended. But with around one in 11 nhs posts vacant, it will be tricky to find enough radiologists and general practitioners to make a difference.

Next, prevention. Individuals bear responsibility for their own decisions but public-health interventions, from vaccines to anti-smoking and weight-loss programmes, can improve things. They also provide good value for money. One study found that it cost nearly four times as much to gain an extra year of good health via clinical interventions than through public-health programmes. Yet funding for the public-health grant, which is allocated to local authorities by central government and amounts to a mere 2% of the nhs budget, has been cut in real terms in recent years.

Ultimately the greatest improvements will come from raising the living standards of the poor. Their lower life expectancy has many causes, from less money to spend on home insulation or nutritious food, to the stress of financial insecurity. One useful long-term thing the government can do is help improve the country’s dreadful record on productivity by liberalising planning and devolving fiscal powers to local authorities.

Life after life

The government should also recognise the role that deprivation plays in health. Reweighting funding formulas to benefit general practitioners in the poorest areas would be a good idea. They care for 10% more patients than practices in the richest areas, but receive 7% less cash. And as Jeremy Hunt, the chancellor of the exchequer, prepares his budget for March 15th, he should recognise how spending cuts show up in other areas. The data show that life expectancy was worst affected in the places with the largest relative declines in housing services and adult social-care spending between 2009 and 2019.

In its covid response, Britain went to extraordinary lengths to prevent its citizens from suffering an early death. The pandemic may be over, but that job is nowhere near complete.

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It's very simple Alan, your NIH, just like ours here are corrupt and involved in suppressing vitamin D benefits from the public. Some years ago the EU had a rash of infant deaths and they blamed it on vitamin D fortification and they over reacted and stopped fortification.

Vitamin D food fortification in European countries: the underused potential to prevent cancer deaths

Vitamin D food fortification in European countries: the underused potential to prevent cancer deaths

Background: Meta-analyses of randomized controlled trials have shown that vitamin D supplementation reduces cancer mortality by 13%. Vitamin D fortification of…

www.ncbi.nlm.nih.gov

Does fortification of staple foods improve vitamin D intakes and status of groups at risk of deficiency? A United Kingdom modeling study

Does fortification of staple foods improve vitamin D intakes and status of groups at risk of deficiency? A United Kingdom modeling study

ABSTRACT. Background: More than one-fifth of the United Kingdom population has poor vitamin D status (serum 25-hydroxyvitamin D [25(OH)D] concentration <25 n

academic.oup.com

Alarmingly high' vitamin D deficiency in the United Kingdom

'Alarmingly high' vitamin D deficiency in the United Kingdom

Over 50 per cent of Asians living in the UK are severely deficient in vitamin D, leaving them more vulnerable to respiratory infections such as COVID-19 and…

www.sciencedaily.com

Treason of the Science Journals

How Anthony Fauci manufactured consensus on the origins of COVID-19 with the help of science writers and the media

Treason of the Science Journals

How Anthony Fauci manufactured consensus on the origins of COVID-19 with the help of science writers and the media

www.tabletmag.com

At the government level, pandemic preparedness is as much about protecting critical supply chains as it is about administering medical treatments. What the COVID-19 pandemic showed is that the flow of information, which may be the single most vital resource in the supply chain, is utterly broken. In many cases, it was actively undermined by senior public health officials including the former chief medical adviser to the president, Dr. Anthony Fauci.

New emails released in a congressional probe show that Fauci helped direct the publication of “The Proximal Origin of SARS-CoV-2,” an influential scientific paper published in Nature Medicine on March 17, 2020, that claimed COVID-19 could not have leaked from a laboratory. Fauci then cited the paper—in effect quoting himself, since he coordinated the article behind the scenes and was given final approval before it published—as if it was an independent source corroborating his assertions that COVID could only have come from a bat and not from a lab.

“There was a study recently that we can make available to you, where a group of highly qualified evolutionary virologists looked at the sequences there and the sequences in bats as they evolve,” Fauci said at a presidential briefing on April 17, 2020, exactly one month after “Proximal Origin” was published. “And the mutations that it took to get to the point where it is now is totally consistent with a jump of a species from an animal to a human.”

But why would Fauci go to so much trouble to control the information surrounding the origins of the virus while sending the message to Americans that the idea that COVID had come from a lab was a conspiracy theory? And why would science journalists and peer-reviewed science publications go along with the effort?

Long Covid patients face medical debt after insurance denies claims

Millions in the U.S. are out of work because of severe long Covid symptoms, and many are struggling to pay their medical bills. Here's how to navigate insurance coverage.

Long Covid treatment can lead to debt after insurance denies claims

Millions in the U.S. are out of work because of severe long Covid symptoms, and many are struggling to pay their medical bills. Here's how to navigate…

www.nbcnews.com

When Maness submitted the testing to her insurance — Anthem Blue Cross — the provider deemed the testing medically unnecessary and declined to cover the cost. She’s now on the hook for the medical bills, which have already cost her more than $10,000 out of pocket.

“I’m kind of at the point sadly where I’ve just given up,” said Maness, a Ph.D. student in Sacramento, California. Many of her insurance appeals have been denied. “I don’t have the mental bandwidth to even battle this anymore, because it’s become clear that it is most likely going to be unsuccessful.”

Maness is among several long Covid patients in the United States interviewed by NBC News who say their insurance providers are declining to provide coverage related to their illness.

They fill their pockets with your premium payments and refuse to pay for treatment recieved. Hmmmmmm

Another way to profit off of Covid!!!!!!

This is an interesting article. This age group coincides with the explosion in cable TV and video games. It also coincides with parents starting to micromanage every aspect of our children's lives. To put it simply they are vitamin d deficient!!!

Millennials are racking up more chronic health conditions compared to other generations: study

Millennials are racking up more chronic health conditions compared to other generations: study

Millennials are seeking more professional help for health issues than ever, a new study revealed.

nypost.com

Millennials are seeking more professional help for health issues than ever, a new study revealed.

Conducted by United Healthcare and Health Action Council, the study explored factors and claims data from policyholders ages 27 to 42 — a total of 126,000 individuals.

It compared current data from April 2021 through March 2022 to historical data dating back to 2012. The findings were presented in the groups’ sixth annual white paper.

mRNA 'Vaccines' Contain Nanotechnology Formulated with Graphene Oxide

mRNA 'Vaccines' Contain Nanotechnology Formulated with Graphene Oxide

In my recent interview with Stew Peters, I review the confidential Pfizer research and development trade secret document for the invention of Pfizer’s mRNA…

karenkingston.substack.com

Global citizens were told that the vaccines contained ‘mRNA lipids’ because no one of sound mind would agree to be injected with electromagnetic nanotechnology devices.

Even Dr. Robert Malone, the inventor of mRNA vaccines, often boasts how he invented the cationic liposome nanotechnology. Cationic liposomes are electronic nanotechnologies which are now dubbed as one of the ‘lipid nanoparticles’ in the COVID-19 mRNA ‘vaccines.’

Cleveland Clinic Study Destroys mRNA Injections Biggest Lie

Cleveland Clinic Study Destroys mRNA Injections Biggest Lie

Cleveland Clinic study of 51,011 employees, Pfizer post-hoc analysis, and the FDA approval documents confirm mRNA biological injections cause COVID-19…

karenkingston.substack.com

Currently the CDC, FDA, NIH, and most government officials, healthcare professionals and mainstream media, are promoting the false claim that the COVID-19 mRNA biological injections prevent hospitalizations and severe disease. This ‘spin’ was born from the harsh reality that the COVID-19 mRNA biological injections do not prevent SARS-CoV-2 infection or transmission.

If a ‘Vaccine” Can’t Prevent Viral Infection then It Can’t Prevent Severe Disease Either. This is Common Sense. No Expert Opinion Required.

My question is, ‘If a vaccine can not reduce the risk for a viral infection, how can it reduce the risk for the severe disease that is caused by that viral infection?’

The answer is it can’t.

Quote from Fm1

mRNA 'Vaccines' Contain Nanotechnology Formulated with Graphene Oxide

https://karenkingston.substack…es-contain-nanotechnology

Global citizens were told that the vaccines contained ‘mRNA lipids’ because no one of sound mind would agree to be injected with electromagnetic nanotechnology devices.

Even Dr. Robert Malone, the inventor of mRNA vaccines, often boasts how he invented the cationic liposome nanotechnology. Cationic liposomes are electronic nanotechnologies which are now dubbed as one of the ‘lipid nanoparticles’ in the COVID-19 mRNA ‘vaccines.’

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I have listened to her statements for a good while now (18 months perhaps?). She makes a lot of claims from her analysis of the patents and prior patents. One may agree or disagree with her, but she certainly has done a lot of surveying. I once listened a 2 hours presentation of her that was really well supported in her assesment of the patents, no matter how painfull her conclusions are. Anyway, I can only be sure that anyone that has not bothered to do the same level of deep diving, probably will dismiss all what Karen Kingston says as lunatic ravings.

Dr Campbell said that the Lancet has a good record..

its a bit difficult to remain clean and pure in todays complex pharmacomedico ' system'

where the BigPharma $ muddies the water on a daily basis.

but "Sinophobia"? Really?

"It’s not just the scientists and health workers of China that the Lancet has praised. In May last year, its editor-in-chief Richard Horton appeared on the state-owned broadcaster China Central Television to praise how ‘tremendously decisively’ the Chinese Communist party had handled the pandemic. He also penned multiple editorials about China, including one entitled ‘Covid-19 and the Dangers of Sinophobia’.

Richard Charles Horton OBE FRCP FMedSci

How the Lancet lost our trust

Right from its first issue in 1823, the Lancet was more than just an ordinary medical journal. Its founding editor, the dyspeptic surgeon and coroner Thomas…

www.spectator.co.uk

Could this be behind the rise in violet crime around the world?

Vaccine-Induced Psychosis as an Etiology to Consider in the Age of COVID-19

https://www.psychiatrist.com/p…0deserves%20consideration.

This is a perplexing case of a patient with no psychiatric, trauma, family history, or personality pathology who received the mRNA-1273 (Moderna) coronavirus disease 2019 (COVID-19) vaccine and within a month developed an acute paranoid state.

Violent crime rises as police struggle

Drop in nationwide homicide rate belies brutality on streets

Violent crime rises as police struggle

Homicides dipped slightly in 2022, but violent crimes such as rape, robbery and assault remain dramatically higher than before the COVID-19 pandemic, The…

www.washingtontimes.com

Another loud-mouthed ivermectard ‘influencer’ removes himself from the gene pool… His last words were:

“HAPPY FRIDAY ALL YOU POISONOUS HORSE PASTE EATING SURVIVORS !!!”  

He was found dead three hours later. Moderators on the forum where he pushed his daft ideas onto other dupes, made light of the situation with this weird quote:

“Though it was obvious that Danny had the biggest heart, it was unbeknownst to him that his heart was quite literally overworking and overgrowing beyond its capacity, nearly doubled in size from what it should have been.”

In response to the death notice, another moron member of his forum had this to say:

“My wife has been taking ivermectin for 3 months… Today she has a migraine, vomiting and severe stomach pain. Does anyone have any ideas how to help…?”

Errrrmmmm…. Nope, all out of ideas here. Maybe give her some more ivermectin?

An Ivermectin Influencer Died. Now His Followers Are Worried About Their Own ‘Severe’ Symptoms.

Danny Lemoi took a daily dose of veterinary-grade ivermectin and told his thousands of followers to give the drug to children. He died of a common side effect…

www.vice.com

There’s some more choice quotes in the article - again coming from people who are genuinely befuddled by the wide range of strange symptoms they have been suffering from since they started guzzling down the horse paste on a regular basis.

Apparently, a small minority of members managed to correctly put the wooden triangle into the triangle-shaped hole, before being shouted down by the rest of the members for “spreading misinformation”.

Classic!