Innate Immune Suppression by SARS-CoV-2 mRNA Vaccinations: The role of G-quadruplexes, Exosomes, and MicroRNAs
https://www.trialsitenews.com/…es-and-micrornas-0626cb33
Stephanie Seneff
Opinion Article
The SARS-CoV-2 messenger RNA vaccines are based on a revolutionary new technology that involves injecting into the arm muscle many molecules of the mRNA sequence that codes for the spike glycoprotein, a major protein in the envelope of the SARS-CoV-2 virus. This protein is arguably the most toxic part of the virus. It is what allows the virus to bind to ACE2 receptors in cells, disabling them. The spike protein has a novel furin cleavage site that allows it to be snipped in half, releasing a segment called S1, which can then travel all around the vascular system and the lymph system. S1 carries the ACE2-binding site, and, when S1 by itself binds to an ACE2 receptor, the receptor becomes disabled. This has a profound impact, leading to excessive levels of angiotensin II and an acute inflammatory response, causing a wide range of symptoms and damage in both the heart and the brain. While the vaccines can only produce the spike protein and not the complete virus, this protein has profound toxic effects, systemically.
Importantly, the mRNA that is in the vaccine is very different from the original viral mRNA sequence. Whereas the virus enters via the nose and lungs, the vaccine is injected past both the mucosal barrier and the vascular barrier. Researchers developing this technology were concerned about the RNA being attacked and broken down by the immune system before it had a chance to produce protein. A major breakthrough was when they discovered they could replace all of the uridines in the sequence with methylpseudouridine, a modification that makes the RNA very sturdy and difficult to break down. Also, a change in the genetic code introduced a pair of prolines causing the S1 subunit to stay stuck on the ACE2 receptors, disabling them. They also “humanized” the mRNA such that its viral origins are disguised, and they performed “codon optimization,” greatly altering the mRNA sequence from the original codons used by the virus, swapping in codons that are known to be more efficient at rapidly producing a steady stream of spike proteins.
The muscle cells that take up the lipid particles encasing the mRNA immediately start synthesizing spike protein at a rapid and sustained pace. These cells display the newly synthesized spike proteins on their surface, and this draws in immune cells, especially dendritic cells, which also take up the vaccine lipid nanoparticles. Dendritic cells do not express ACE2 receptors, so they are resistant to viral infection, but not to the vaccine. They too start synthesizing spike protein, which is neurotoxic and which has prion-like features. They quickly enter the lymph system, carrying their toxic load into the lymph nodes and beyond, ultimately congregating in the spleen and liver.
Germinal centers in the spleen become the playing field where the dendritic cells release the spike protein they are continually producing into the external milieu, packaged up within exosomes, small lipid particles that serve as a communication network for the organism. These activities draw B-cells and T-cells to the scene, and they get to work perfecting antibodies that exactly match portions of the spike protein. The primary goal of the vaccine is to get the immune system to produce abundant antibodies to the spike protein, and indeed they perform very well in this goal.
The immune cells are compelled to release exosomes in order to rid themselves of the toxic spike protein rapidly accumulating in their cytoplasm. Unfortunately, the exosomes travel very well along nerve fibers. They travel from the germinal centers in the spleen up the splanchnic nerve to the celiac ganglia and beyond along the vagus nerve to eventually reach major organs of the body: the liver, the lungs, the heart, and the brain. At these destinations, the spike-containing exosomes are taken up by resident immune cells, which then activate an inflammatory cascade causing localized tissue damage.
A seminal paper showed experimentally that vaccine transfected human cells take up the lipid nanoparticles and release exosomes containing not only spike protein but also certain specific microRNAs that have a profound negative effect on innate immunity. MicroRNAs are small non-coding RNA sequences that have powerful abilities to suppress expression of specific proteins. The two miRNAs that these researchers found in the exosomes suppress two proteins that play a critical role in executing the type I interferon response. Type I interferons activate an early alarm system that defends the organism against any kind of infection as well as cancer. Suppression of type I interferon can cause latent viruses like herpes and varicella to become activated and can cause cancer to come out of remission.
Perhaps most alarming is the potential for the vaccine to cause neurodegenerative disease. There is a motif that is characteristic of prion proteins called the GxxxG motif (two glycine residues separated by three wildcards). The human prion protein has 14 GxxxGs, and amyloid-beta, the protein linked to Alzheimer’s disease, has 4. Most proteins have none. The SARS-CoV-2 spike protein includes 7 GxxxGs. There is a fascinating structural motif in DNA and RNA called a “guanine quadruplex” (G4) which has been found to play multiple profound roles in regulating transcription and protein expression. Significantly, studies have shown that the prion protein binds to G4s in its own RNA, and this can trigger it to misfold into its toxic shape. What is very disturbing is that the RNA in the vaccine has been engineered via codon optimization to have many more guanines than the original virus version of the RNA. This means that there is a greater potential for the spike proteins to misfold after binding to G4s that are enriched in the vaccine mRNA.
Our paper provides several tables showing the percentage of various reactions associated with COVID-19 vaccines compared to all vaccines in 2021, according to the vaccine adverse event reporting system (VAERS). First of all, we calculated that overall there were 27 times as many reports for COVID-19 vaccines as would have been expected if their adverse effect profile had been comparable to that of the flu vaccine. There were over 200,000 events in 2021 linking COVID vaccines to diverse symptoms that are commonly associated with inflammation in major nerves such as the vagus nerve, the cochlea, the facial nerve, and the trigeminal nerve. These accounted for more than 97% of all events linking any vaccine in 2021 with these symptoms. Over 8,000 reactions indicated heart damage linked to COVID-19 vaccines, including myocarditis, heart failure, arrhythmias, and heart attack. These represented nearly 98% of all the reports for heart problems linked to any vaccine. There were over 10,000 cases of thrombosis or pulmonary embolism, accounting for nearly 99% of the total reports for these symptoms. The total counts for symptoms related to liver disease, neurodegenerative diseases, and cancer were much lower, but COVID-19 vaccines accounted for over 97% of the liver-related symptoms, nearly 98% of the cases of memory impairment, 99.5% of cases of anosmia (loss of smell), and over 97% of reports of cancer of specific organs. It is widely acknowledged that VAERS events are vastly under-reported